› 3jqy

X-ray diffraction
1.7Å resolution

Crystal Structure of the polySia specific acetyltransferase NeuO

Released:
DOI: 10.2210/pdb3jqy/pdb
PDB entry 3jqy coloured by chain, front view.
PDB entry 3jqy coloured by chain, side view.
PDB entry 3jqy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure analysis of the polysialic acid specific O-acetyltransferase NeuO.
Schulz EC, Bergfeld AK, Ficner R, Mühlenhoff M
PLoS One 6 e17403 (2011)
PMID: 21390252

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + an alpha-2,8-linked polymer of sialic acid = CoA + polysialic acid acetylated at O-7 or O-9
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polysialic acid O-acetyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 252 amino acids
Theoretical weight: 28.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A1ADJ6 (Residues: 66-307; Coverage: 79%)
Gene names: APECO1_4217, neuO
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

2 bound ligands:
Ligand PEG 2 x PEG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 62.833Å b: 88.366Å c: 72.996Å
α: 90° β: 106.62° γ: 90°
R-values:
R R work R free
0.167 0.166 0.195
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules.
Cress et al. (2014)
2 more

2 mentions without citation

AUSPEX: a graphical tool for X-ray diffraction data analysis.
Thorn et al. (2017)
1 more