› 3h09

X-ray diffraction
1.75Å resolution

The structure of Haemophilus influenzae IgA1 protease

Released:
DOI: 10.2210/pdb3h09/pdb
PDB entry 3h09 coloured by chain, front view.
PDB entry 3h09 coloured by chain, side view.
PDB entry 3h09 coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Active-site gating regulates substrate selectivity in a chymotrypsin-like serine protease the structure of haemophilus influenzae immunoglobulin A1 protease.
Johnson TA, Qiu J, Plaut AG, Holyoak T
J Mol Biol 389 559-74 (2009)
PMID: 19393662

Function and Biology Details

Reaction catalysed: 
Cleavage of immunoglobulin A molecules at certain Pro bonds in the hinge region. No small molecule substrates are known.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin A1 protease Chains: A, B
Molecule details ›
Chains: A, B
Length: 989 amino acids
Theoretical weight: 108.8 KDa
Source organism: Haemophilus influenzae
UniProt:
  • Canonical: P44969 (Residues: 26-1014; Coverage: 59%)
Gene names: HI_0990, iga, iga1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ACT 4 x ACT
Ligand MLA 5 x MLA
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 94.385Å b: 131.872Å c: 111.813Å
α: 90° β: 113.11° γ: 90°
R-values:
R R work R free
0.162 0.161 0.19

Quick links

Citations

12 review citations

Structure and function relationships in IgA.
Woof et al. (2011)
11 more

12 mentions without citation

Serine protease autotransporters of enterobacteriaceae (SPATEs): biogenesis and function.
Dautin N. (2010)
11 more