› 3cvr

X-ray diffraction
2.8Å resolution

Crystal structure of the full length IpaH3

Released:
DOI: 10.2210/pdb3cvr/pdb
PDB entry 3cvr coloured by chain, front view.
PDB entry 3cvr coloured by chain, side view.
PDB entry 3cvr coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 301
Primary publication:
Structure of a Shigella effector reveals a new class of ubiquitin ligases.
Zhu Y, Li H, Hu L, Wang J, Zhou Y, Pang Z, Liu L, Shao F
Nat Struct Mol Biol 15 1302-8 (2008)
PMID: 18997779

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-182957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase ipaH3 Chain: A
Molecule details ›
Chain: A
Length: 571 amino acids
Theoretical weight: 65.51 KDa
Source organism: Shigella flexneri 2a str. 301
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q83RJ4 (Residues: 1-571; Coverage: 100%)
Gene names: SF1383, ipaH3
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A, PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P41212
Unit cell:
a: 154.19Å b: 154.19Å c: 85.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.251 0.277
Expression system: Escherichia coli BL21

Quick links

Citations

38 review citations

Common themes in the design and function of bacterial effectors.
Galán JE. (2009)
37 more

15 mentions without citation

Hijacking the host ubiquitin pathway: structural strategies of bacterial E3 ubiquitin ligases.
Hicks et al. (2010)
14 more