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Protecting role of cosolvents in protein denaturation by SDS: a structural study.

Michaux C, Pouyez J, Wouters J, Privé GG
BMC Struct Biol 8 29 (2008)
Cited: 13 times
EuropePMC logo PMID: 18522744

Abstract

Background

Recently, we reported a unique approach to preserve the activity of some proteins in the presence of the denaturing agent, Sodium Dodecyl Sulfate (SDS). This was made possible by addition of the amphipathic solvent 2,4-Methyl-2-PentaneDiol (MPD), used as protecting but also as refolding agent for these proteins. Although the persistence of the protein activity in the SDS/MPD mixture was clearly established, preservation of their structure was only speculative until now.

Results

In this paper, a detailed X-ray study addresses the pending question. Crystals of hen egg-white lysozyme were grown for the first time in the presence of MPD and denaturing concentrations of SDS. Depending on crystallization conditions, tetragonal crystals in complex with either SDS or MPD were collected. The conformation of both structures was very similar to the native lysozyme and the obtained complexes of SDS-lysozyme and MPD-lysozyme give some insights in the interplay of protein-SDS and protein-MPD interactions.

Articles - 3b72 mentioned but not cited (2)

  1. Protecting role of cosolvents in protein denaturation by SDS: a structural study. Michaux C, Pouyez J, Wouters J, Privé GG. BMC Struct Biol 8 29 (2008)
  2. Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol. Stauber M, Jakoncic J, Berger J, Karp JM, Axelbaum A, Sastow D, Buldyrev SV, Hrnjez BJ, Asherie N. Acta Crystallogr D Biol Crystallogr 71 427-441 (2015)


Articles citing this publication (11)

  1. PagP crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler. Cuesta-Seijo JA, Neale C, Khan MA, Moktar J, Tran CD, Bishop RE, Pomès R, Privé GG. Structure 18 1210-1219 (2010)
  2. Complexes of native ubiquitin and dodecyl sulfate illustrate the nature of hydrophobic and electrostatic interactions in the binding of proteins and surfactants. Shaw BF, Schneider GF, Arthanari H, Narovlyansky M, Moustakas D, Durazo A, Wagner G, Whitesides GM. J Am Chem Soc 133 17681-17695 (2011)
  3. Towards a universal method for protein refolding: the trimeric beta barrel membrane Omp2a as a test case. Roussel G, Perpète EA, Matagne A, Tinti E, Michaux C. Biotechnol Bioeng 110 417-423 (2013)
  4. Effect of disaccharides on the stabilization of bovine trypsin against detergent and autolysis. Prasad S, Roy I. Biotechnol Prog 26 627-635 (2010)
  5. Structural and thermodynamic studies of the tobacco calmodulin-like rgs-CaM protein. Makiyama RK, Fernandes CA, Dreyer TR, Moda BS, Matioli FF, Fontes MR, Maia IG. Int J Biol Macromol 92 1288-1297 (2016)
  6. Using Hemolysis as a Novel Method for Assessment of Cytotoxicity and Blood Compatibility of Decellularized Heart Tissues. Momtahan N, Panahi T, Poornejad N, Stewart MG, Vance BR, Struk JA, Castleton AA, Roeder BL, Sukavaneshvar S, Cook AD. ASAIO J 62 340-348 (2016)
  7. Unravelling the mechanisms of a protein refolding process based on the association of detergents and co-solvents. Michaux C, Roussel G, Lopes-Rodrigues M, Matagne A, Perpète EA. J Pept Sci 22 485-491 (2016)
  8. Purification and characterization of an amyloglucosidase from an ericoid mycorrhizal fungus (Leohumicola incrustata). Adeoyo OR, Pletschke BI, Dames JF. AMB Express 8 154 (2018)
  9. Refolding of SDS-denatured proteins using amphipathic cosolvents and osmolytes. Roussel G, Tinti E, Perpète E, Michaux C. Curr Protoc Protein Sci Chapter 28 Unit28.5 (2013)
  10. 2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization. Kinzel J, Sauer DF, Bocola M, Arlt M, Mirzaei Garakani T, Thiel A, Beckerle K, Polen T, Okuda J, Schwaneberg U. Beilstein J Org Chem 13 1498-1506 (2017)
  11. Variation in chicken populations may affect the enzymatic activity of lysozyme. Downing T, O'Farrelly C, Bhuiyan AK, Silva P, Naqvi AN, Sanfo R, Sow RS, Podisi B, Hanotte O, Bradley DG. Anim Genet 41 213-217 (2010)


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