› 3te8

X-ray diffraction
1.7Å resolution

Crystal structure of risS periplasmic domain at low pH dimer with N-termini ordered

Released:
DOI: 10.2210/pdb3te8/pdb
PDB entry 3te8 coloured by chain, front view.
PDB entry 3te8 coloured by chain, side view.
PDB entry 3te8 coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Entry authors: Edwards TE, Abendroth J, Labiuk SL, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.54 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JRA3 (Residues: 30-151; Coverage: 27%)
Gene names: BURPS1710b_2508, risS
Sequence domains: Periplasmic domain of Sensor histidine kinase RisS
Structure domains: Sensor histidine kinase RisS, periplasmic domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 41.06Å b: 56.02Å c: 46.81Å
α: 90° β: 96.03° γ: 90°
R-values:
R R work R free
0.184 0.182 0.228
Expression system: Escherichia coli

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