› 3gj1

X-ray diffraction
1.8Å resolution

Non photoactivated state of PA-GFP

Released:
DOI: 10.2210/pdb3gj1/pdb
PDB entry 3gj1 coloured by chain, front view.
PDB entry 3gj1 coloured by chain, side view.
PDB entry 3gj1 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structure and mechanism of the photoactivatable green fluorescent protein.
Henderson JN, Gepshtein R, Heenan JR, Kallio K, Huppert D, Remington SJ
J Am Chem Soc 131 4176-7 (2009)
PMID: 19278226

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 229 amino acids
Theoretical weight: 25.98 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand CL 5 x CL
1 modified residue:
Ligand CRO 4 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 84.515Å b: 86.721Å c: 144.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.252
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
16 more

2 mentions without citation

Unified Model for Photophysical and Electro-Optical Properties of Green Fluorescent Proteins.
Lin et al. (2019)
1 more