X-ray diffraction
3.41Å resolution

Crystal Structure Of Human G alpha i1 Bound To A Designed Helical Peptide Derived From The Goloco Motif Of RGS14


Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P63096 (Residues: 30-354; Coverage: 92%)
Gene name: GNAI1
Structure domains:
Regulator of G-protein signaling 14 Chains: C, D
Molecule details ›
Chains: C, D
Length: 40 amino acids
Theoretical weight: 4.7 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: O43566 (Residues: 497-517; Coverage: 4%)
Gene name: RGS14

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I23
Unit cell:
a: 265.261Å b: 265.261Å c: 265.261Å
α: 90° β: 90° γ: 90°
R R work R free
0.223 0.223 0.244
Expression systems:
  • Escherichia coli BL21
  • Not provided