› 2rgt

X-ray diffraction
2.05Å resolution

Crystal Structure of Lhx3 LIM domains 1 and 2 with the binding domain of Isl1

Released:
DOI: 10.2210/pdb2rgt/pdb
PDB entry 2rgt coloured by chain, front view.
PDB entry 2rgt coloured by chain, side view.
PDB entry 2rgt coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes.
Bhati M, Lee C, Nancarrow AL, Lee M, Craig VJ, Bach I, Guss JM, Mackay JP, Matthews JM
EMBO J 27 2018-29 (2008)
PMID: 18583962

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin gene enhancer protein ISL-1; LIM/homeobox protein Lhx3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P50481 (Residues: 28-153; Coverage: 32%)
  • Canonical: P61372 (Residues: 139-143, 144-155, 156-168; Coverage: 9%)
Gene names: Isl1, Lhx3, Lim-3, Lim3, Plim
Sequence domains: LIM domain
Structure domains: Cysteine Rich Protein

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 119.494Å b: 62.218Å c: 51.885Å
α: 90° β: 91.61° γ: 90°
R-values:
R R work R free
0.214 0.211 0.254
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Macromolecular NMR spectroscopy for the non-spectroscopist.
Kwan et al. (2011)
4 more

11 mentions without citation

Linkers in the structural biology of protein-protein interactions.
Reddy Chichili et al. (2013)
10 more