2hos

X-ray diffraction
1.9Å resolution

Phage-Selected Homeodomain Bound to Unmodified DNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Segmentation polarity homeobox protein engrailed Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.52 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P02836 (Residues: 453-513; Coverage: 11%)
Gene names: CG9015, en
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*TP*TP*TP*TP*GP*CP*CP*AP*TP*GP*TP*AP*AP*TP*CP*CP*CP*CP*GP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
5'-D(*AP*TP*CP*CP*GP*GP*GP*GP*AP*TP*TP*AP*CP*AP*TP*GP*GP*CP*AP*AP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.5 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 127.097Å b: 45.025Å c: 73.081Å
α: 90° β: 118.41° γ: 90°
R-values:
R R work R free
0.225 0.223 0.257
Expression systems:
  • Escherichia coli BL21
  • Not provided