› 1tku

X-ray diffraction
1.66Å resolution

Crystal Structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans in complex with Ribulose-5-phosphate

Released:
DOI: 10.2210/pdb1tku/pdb
PDB entry 1tku coloured by chain, front view.
PDB entry 1tku coloured by chain, side view.
PDB entry 1tku coloured by chain, top view.
Source organism: Candida albicans SC5314
Primary publication:
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans.
Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M
J Mol Biol 341 1085-96 (2004)
PMID: 15328619

Function and Biology Details

Reaction catalysed: 
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3,4-dihydroxy-2-butanone 4-phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 22.68 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5A3V6 (Residues: 1-204; Coverage: 100%)
Gene names: CAALFM_C112360CA, CaO19.12693, CaO19.5228, RIB3
Sequence domains: 3,4-dihydroxy-2-butanone 4-phosphate synthase
Structure domains: DHBP synthase

Ligands and Environments

1 bound ligand:
Ligand 5RP 2 x 5RP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 40.2Å b: 47.9Å c: 59.8Å
α: 66.4° β: 72.3° γ: 89.9°
R-values:
R R work R free
0.184 0.184 0.218
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
4 more

2 mentions without citation

Structural basis for competitive inhibition of 3,4-dihydroxy-2-butanone-4-phosphate synthase from Vibrio cholerae.
Islam et al. (2015)
1 more