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Biosynthetic uniform 13C,15N-labelling of zervamicin IIB. Complete 13C and 15N NMR assignment.

Ovchinnikova TV, Shenkarev ZO, Yakimenko ZA, Svishcheva NV, Tagaev AA, Skladnev DA, Arseniev AS
J Pept Sci 9 817-26
Cited: 7 times
EuropePMC logo PMID: 14658801

Abstract

Zervamicin IIB is a member of the alpha-aminoisobutyric acid containing peptaibol antibiotics. A new procedure for the biosynthetic preparation of the uniformly 13C- and 15N-enriched peptaibol is described This compound was isolated from the biomass of the fungus-producer Emericellopsis salmosynnemata strain 336 IMI 58330 obtained upon cultivation in the totally 13C, 15N-labelled complete medium. To prepare such a medium the autolysed biomass and the exopolysaccharides of the obligate methylotrophic bacterium Methylobacillus flagellatus KT were used. This microorganism was grown in totally 13C, 15N-labelled minimal medium containing 13C-methanol and 15N-ammonium chloride as the only carbon and nitrogen sources. Preliminary NMR spectroscopic analysis indicated a high extent of isotope incorporation (> 90%) and led to the complete 13C- and 15N-NMR assignment including the stereospecific assignment of Aib residues methyl groups. The observed pattern of the structurally important secondary chemical shifts of 1H(alpha), 13C=O and 13C(alpha) agrees well with the previously determined structure of zervamicin IIB in methanol solution.

Articles - 1r9u mentioned but not cited (1)

  1. Peptaibol zervamicin IIb structure and dynamics refinement from transhydrogen bond J couplings. Shenkarev ZO, Balashova TA, Yakimenko ZA, Ovchinnikova TV, Arseniev AS. Biophys. J. 86 3687-3699 (2004)


Articles citing this publication (6)

  1. Antiamoebin I in methanol solution: rapid exchange between right-handed and left-handed 3(10)-helical conformations. Shenkarev ZO, Paramonov AS, Nadezhdin KD, Bocharov EV, Kudelina IA, Skladnev DA, Tagaev AA, Yakimenko ZA, Ovchinnikova TV, Arseniev AS. Chem. Biodivers. 4 1219-1242 (2007)
  2. High stability of the hinge region in the membrane-active peptide helix of zervamicin: paramagnetic relaxation enhancement studies. Shenkarev ZO, Paramonov AS, Balashova TA, Yakimenko ZA, Baru MB, Mustaeva LG, Raap J, Ovchinnikova TV, Arseniev AS. Biochem. Biophys. Res. Commun. 325 1099-1105 (2004)
  3. Neuroleptic properties of the ion-channel-forming peptaibol zervamicin: locomotor activity and behavioral effects. Ovchinnikova TV, Levitskaya NG, Voskresenskaya OG, Yakimenko ZA, Tagaev AA, Ovchinnikova AY, Murashev AN, Kamenskii AA. Chem. Biodivers. 4 1374-1387 (2007)
  4. Peptaibol antiamoebin I: spatial structure, backbone dynamics, interaction with bicelles and lipid-protein nanodiscs, and pore formation in context of barrel-stave model. Shenkarev ZO, Paramonov AS, Lyukmanova EN, Gizatullina AK, Zhuravleva AV, Tagaev AA, Yakimenko ZA, Telezhinskaya IN, Kirpichnikov MP, Ovchinnikova TV, Arseniev AS. Chem. Biodivers. 10 838-863 (2013)
  5. The peptaibol antibiotic zervamicin displays neurotropic activity. Ovchinnikova TV, Murashev AN. Dokl Biochem Biophys 414 146-148 (2007)
  6. Trans and surface membrane bound zervamicin IIB: 13C-MAOSS-NMR at high spinning speed. Raap J, Hollander J, Ovchinnikova TV, Swischeva NV, Skladnev D, Kiihne S. J. Biomol. NMR 35 285-293 (2006)


Related citations provided by authors (1)

  1. NMR structure of the channel-former zervamicin IIB in isotropic solvents.. Balashova TA, Shenkarev ZO, Tagaev AA, Ovchinnikova TV, Raap J, Arseniev AS FEBS Lett 466 333-6 (2000)

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