› 1jf2

X-ray diffraction
1.72Å resolution

Crystal Structure of W92F obelin mutant from Obelia longissima at 1.72 Angstrom resolution

Released:
DOI: 10.2210/pdb1jf2/pdb
PDB entry 1jf2 coloured by chain, front view.
PDB entry 1jf2 coloured by chain, side view.
PDB entry 1jf2 coloured by chain, top view.
Source organism: Obelia longissima
Primary publication:
Violet bioluminescence and fast kinetics from W92F obelin: structure-based proposals for the bioluminescence triggering and the identification of the emitting species.
Vysotski ES, Liu ZJ, Markova SV, Blinks JR, Deng L, Frank LA, Herko M, Malikova NP, Rose JP, Wang BC, Lee J
Biochemistry 42 6013-24 (2003)
PMID: 12755603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Obelin Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.22 KDa
Source organism: Obelia longissima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27709 (Residues: 1-195; Coverage: 100%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CZH 1 x CZH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 53.449Å b: 53.449Å c: 144.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.232 0.274
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Shining Light on the Secreted Luciferases of Marine Copepods: Current Knowledge and Applications.
Markova et al. (2019)
3 more

2 mentions without citation

All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin.
Deng et al. (2005)
1 more