› 1j1m

X-ray diffraction
1.5Å resolution

Ricin A-Chain (Recombinant) at 100K

Released:
DOI: 10.2210/pdb1j1m/pdb
PDB entry 1j1m coloured by chain, front view.
PDB entry 1j1m coloured by chain, side view.
PDB entry 1j1m coloured by chain, top view.
Source organism: Ricinus communis
Entry authors: Watanabe K, Motoshima H

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.07 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P41212
Unit cell:
a: 67.6Å b: 67.6Å c: 141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.213
Expression system: Escherichia coli

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