7nz6

X-ray diffraction
1.4Å resolution

14-3-3 sigma with RelA/p65 binding site pS45 and covalently bound TCF521-125

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-152152 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Transcription factor p65 Chain: P
Molecule details ›
Chain: P
Length: 13 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q04206 (Residues: 39-51; Coverage: 2%)
Gene names: NFKB3, RELA

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 82.565Å b: 112.612Å c: 62.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.213
Expression systems:
  • Escherichia coli
  • Not provided