› 7eb9

X-ray diffraction
3.2Å resolution

The structure of the A20-binding inhibitor of NF-kB 1 in complex with tetra-ubiquitin

Released:
DOI: 10.2210/pdb7eb9/pdb
PDB entry 7eb9 coloured by chain, front view.
PDB entry 7eb9 coloured by chain, side view.
PDB entry 7eb9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and Biochemical Basis for Higher-Order Assembly between A20-Binding Inhibitor of NF-κB 1 (ABIN1) and M1-Linked Ubiquitins.
Hong JY, Lin SC, Kuo BJ, Lo YC
J Mol Biol 433 167116 (2021)
PMID: 34161781

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143376 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 34.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 381-684; Coverage: 44%)
Gene name: UBC
Sequence domains: Ubiquitin family
TNFAIP3-interacting protein 1 Chains: B, C
Molecule details ›
Chains: B, C
Length: 99 amino acids
Theoretical weight: 11.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15025 (Residues: 415-513; Coverage: 16%)
Gene names: KIAA0113, NAF1, TNIP1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Unit cell:
a: 45.381Å b: 53.777Å c: 240.115Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.216 0.264
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

A20 binding and inhibitor of nuclear factor kappa B (NF-κB)-1 (ABIN-1): a novel modulator of mitochondrial autophagy.
Merline et al. (2023)