› 6y23

X-ray diffraction
2.58Å resolution

DDR1 kinase autoinhibited by its juxtamembrane region

Released:
DOI: 10.2210/pdb6y23/pdb
PDB entry 6y23 coloured by chain, front view.
PDB entry 6y23 coloured by chain, side view.
PDB entry 6y23 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Two-step release of kinase autoinhibition in discoidin domain receptor 1.
Sammon D, Hohenester E, Leitinger B
Proc Natl Acad Sci U S A 117 22051-22060 (2020)
PMID: 32839343

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170636 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epithelial discoidin domain-containing receptor 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 350 amino acids
Theoretical weight: 39.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q08345 (Residues: 566-913; Coverage: 39%)
Gene names: CAK, DDR1, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P6322
Unit cell:
a: 105.307Å b: 105.307Å c: 406.939Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.225 0.27
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Extracellular matrix-induced signaling pathways in mesenchymal stem/stromal cells.
Novoseletskaya et al. (2023)
1 more

1 mention without citation

Structure of LRRK1 and mechanisms of autoinhibition and activation.
Reimer et al. (2023)