› 6w22

Electron Microscopy
3Å resolution

ClpA Engaged1 State bound to RepA-GFP (ClpA Focused Refinement)

Released:
DOI: 10.2210/pdb6w22/pdb
PDB entry 6w22 coloured by chain, front view.
PDB entry 6w22 coloured by chain, side view.
PDB entry 6w22 coloured by chain, top view.
Source organisms:
Primary publication:
Conformational plasticity of the ClpAP AAA+ protease couples protein unfolding and proteolysis.
Lopez KE, Rizo AN, Tse E, Lin J, Scull NW, Thwin AC, Lucius AL, Shorter J, Southworth DR
Nat Struct Mol Biol 27 406-416 (2020)
PMID: 32313240
Related structures: EMD-21522

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142167 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent Clp protease ATP-binding subunit ClpA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 758 amino acids
Theoretical weight: 84.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABH9 (Residues: 1-758; Coverage: 100%)
Gene names: JW0866, b0882, clpA, lopD
Sequence domains:
RepA, green fluorescent protein fusion Chain: X
Molecule details ›
Chain: X
Length: 24 amino acids
Theoretical weight: 2.06 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ATP 8 x ATP
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-21522
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Mitochondrial ClpP serine protease-biological function and emerging target for cancer therapy.
Nouri et al. (2020)
8 more

6 mentions without citation

Recent structural insights into the mechanism of ClpP protease regulation by AAA+ chaperones and small molecules.
Mabanglo et al. (2022)
5 more