› 6sq1

X-ray diffraction
1.49Å resolution

Crystal structure of cAMP-dependent Protein Kinase A (CHO PKA) in complex with Aminofasudil

Released:
DOI: 10.2210/pdb6sq1/pdb
PDB entry 6sq1 coloured by chain, front view.
PDB entry 6sq1 coloured by chain, side view.
PDB entry 6sq1 coloured by chain, top view.
Source organism: Cricetulus griseus
Entry authors: Oebbeke M, Siefker C, Heine A, Klebe G

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 41.11 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25321 (Residues: 1-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
Ligand MPD 2 x MPD
Ligand LRT 1 x LRT
Ligand DMS 2 x DMS
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 52.024Å b: 71.541Å c: 97.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.177
Expression system: Escherichia coli BL21(DE3)

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