› 6qso

X-ray diffraction
1.8Å resolution

mTFP* closed conformation: I197E-Y200H-Y204H mutant for enhanced metal binding

Released:
DOI: 10.2210/pdb6qso/pdb
PDB entry 6qso coloured by chain, front view.
PDB entry 6qso coloured by chain, side view.
PDB entry 6qso coloured by chain, top view.
Source organism: Clavularia sp.
Primary publication:
A Robust and Versatile Host Protein for the Design and Evaluation of Artificial Metal Centers
Fischer J, Renn D, Quitterer F, Radhakrishnan A, Liu M, Makki A, Rajjaka A, Ghoprade S, Rueping M, Arold TA, Groll M, Eppinger J
ACS Catalysis (2019)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein cFP484 Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 25.15 KDa
Source organism: Clavularia sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y3 (Residues: 44-260; Coverage: 82%)
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 34.757Å b: 84.993Å c: 38.497Å
α: 90° β: 112.56° γ: 90°
R-values:
R R work R free
0.156 0.154 0.198
Expression system: Escherichia coli

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