› 6oqk

Solution NMR

SOLUTION STRUCTURE OF THE COMPLEX OF MUTANT VEK50[RH2/AA] AND PLASMINOGEN KRINGLE 2

Released:
DOI: 10.2210/pdb6oqk/pdb
PDB entry 6oqk coloured by chain, ensemble of 10 models, front view.
PDB entry 6oqk coloured by chain, ensemble of 10 models, side view.
PDB entry 6oqk coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
Solution structural model of the complex of the binding regions of human plasminogen with its M-protein receptor from Streptococcus pyogenes.
Yuan Y, Ayinuola YA, Singh D, Ayinuola O, Mayfield JA, Quek A, Whisstock JC, Law RHP, Lee SW, Ploplis VA, Castellino FJ
J Struct Biol 208 18-29 (2019)
PMID: 31301349

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa, higher selectivity than trypsin. Converts fibrin into soluble products.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133233 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Angiostatin Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.17 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00747 (Residues: 179-262; Coverage: 11%)
Gene name: PLG
Sequence domains: Kringle domain
Plasminogen-binding group A streptococcal M-like protein PAM Chain: B
Molecule details ›
Chain: B
Length: 52 amino acids
Theoretical weight: 6.02 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P49054 (Residues: 85-133; Coverage: 14%)
Gene names: emm, pam
Sequence domains: Plasminogen (Pg) ligand in fibrinolytic pathway

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: simulated annealing
Expression systems:
  • Komagataella pastoris
  • Escherichia coli

Quick links

Citations

5 citation in other articles

The M Protein of Streptococcus pyogenes Strain AP53 Retains Cell Surface Functional Plasminogen Binding after Inactivation of the Sortase A Gene.
Russo et al. (2020)
4 more

2 mentions without citation

Solution structural model of the complex of the binding regions of human plasminogen with its M-protein receptor from Streptococcus pyogenes.
Yuan et al. (2019)
1 more