› 6llh

X-ray diffraction
1.99Å resolution

Biphenyl-2,3-diol-soaked resting complex of Oxy and Fd in carbazole 1,9a-dioxygenase

Released:
DOI: 10.2210/pdb6llh/pdb
PDB entry 6llh coloured by chain, front view.
PDB entry 6llh coloured by chain, side view.
PDB entry 6llh coloured by chain, top view.
Source organisms:
Entry authors: Wang YX, Suzuki-Minakuchi C, Nojiri H

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183053 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rieske domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 392 amino acids
Theoretical weight: 44.91 KDa
Source organism: Janthinobacterium sp. J3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84II6 (Residues: 1-384; Coverage: 100%)
Gene name: carAa
Sequence domains:
Ferredoxin CarAc Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 115 amino acids
Theoretical weight: 12.45 KDa
Source organism: Pseudomonas resinovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GI16 (Residues: 1-107; Coverage: 100%)
Gene name: carAc
Sequence domains: Rieske [2Fe-2S] domain

Ligands and Environments

8 bound ligands:
Ligand FE2 3 x FE2
Ligand FES 6 x FES
Ligand BPY 3 x BPY
Ligand EDO 10 x EDO
Ligand PGE 2 x PGE
Ligand ACT 1 x ACT
Ligand PEG 3 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 98.257Å b: 89.793Å c: 105.445Å
α: 90° β: 103.99° γ: 90°
R-values:
R R work R free
0.164 0.162 0.207
Expression system: Escherichia coli BL21(DE3)

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