› 6l33

X-ray diffraction
2Å resolution

Crystal structure of the regulatory domain of MexT, a transcriptional activator in Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb6l33/pdb
PDB entry 6l33 coloured by chain, front view.
PDB entry 6l33 coloured by chain, side view.
PDB entry 6l33 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEFOprN Efflux Pump in Pseudomonas aeruginosa.
Kim S, Kim SH, Ahn J, Jo I, Lee ZW, Choi SH, Ha N
OpenAccess logo Mol Cells 42 850-857 (2019)
PMID: 31722511

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lysR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 212 amino acids
Theoretical weight: 23.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I0Z0 (Residues: 139-347; Coverage: 60%)
Gene names: PA2492, mexT
Sequence domains: LysR substrate binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 65.652Å b: 108.703Å c: 109.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.253
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid.
da Cruz Nizer et al. (2020)
1 more

3 mentions without citation

Anti-Virulence Activity of 3,3'-Diindolylmethane (DIM): A Bioactive Cruciferous Phytochemical with Accelerated Wound Healing Benefits.
Golberg et al. (2022)
2 more