› 6kc6

X-ray diffraction
2.12Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase RNF31 Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 225 amino acids
Theoretical weight: 25.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96EP0 (Residues: 853-1072; Coverage: 21%)
Gene names: RNF31, ZIBRA
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 24 x ZN
Ligand D60 6 x D60
Ligand CL 9 x CL
Ligand GOL 10 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 151.586Å b: 88.793Å c: 104.438Å
α: 90° β: 101.08° γ: 90°
R-values:
R R work R free
0.214 0.211 0.266
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Linear Ubiquitin Code: Its Writer, Erasers, Decoders, Inhibitors, and Implications in Disorders.
Oikawa et al. (2020)
13 more