› 6iwb

X-ray diffraction
2.5Å resolution

Crystal structure of a computationally designed protein (LD3) in complex with BCL-2

Released:
DOI: 10.2210/pdb6iwb/pdb
PDB entry 6iwb coloured by chain, front view.
PDB entry 6iwb coloured by chain, side view.
PDB entry 6iwb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A computationally designed chimeric antigen receptor provides a small-molecule safety switch for T-cell therapy.
Giordano-Attianese G, Gainza P, Gray-Gaillard E, Cribioli E, Shui S, Kim S, Kwak MJ, Vollers S, Corria Osorio AJ, Reichenbach P, Bonet J, Oh BH, Irving M, Coukos G, Correia BE
Nat Biotechnol 38 426-432 (2020)
PMID: 32015549

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135993 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apolipoprotein E Chains: A, C
Molecule details ›
Chains: A, C
Length: 156 amino acids
Theoretical weight: 17.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02649 (Residues: 41-186; Coverage: 49%)
Gene name: APOE
Sequence domains: Apolipoprotein A1/A4/E domain
Apoptosis regulator Bcl-2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 166 amino acids
Theoretical weight: 19.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10415 (Residues: 1-34, 92-207; Coverage: 63%)
Gene name: BCL2
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P65
Unit cell:
a: 129.718Å b: 129.718Å c: 80.291Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.239 0.275
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Navigating CAR-T cells through the solid-tumour microenvironment.
Hou et al. (2021)
23 more

3 mentions without citation

Rational design of small-molecule responsive protein switches.
Shui et al. (2023)
2 more