› 6arg

X-ray diffraction
1.78Å resolution

Aspergillus fumigatus Cytosolic Thiolase: Apo enzyme in complex with rubidium ions

Released:
DOI: 10.2210/pdb6arg/pdb
PDB entry 6arg coloured by chain, front view.
PDB entry 6arg coloured by chain, side view.
PDB entry 6arg coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Structure of Aspergillus fumigatus Cytosolic Thiolase: Trapped Tetrahedral Reaction Intermediates and Activation by Monovalent Cations
Marshall AC, Bond CS, Bruning JB
ACS Catalysis 8(3) 1973-1989 (2018)

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase erg10B, cytosolic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 399 amino acids
Theoretical weight: 41.01 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4WCL5 (Residues: 1-398; Coverage: 100%)
Gene names: AFUA_8G04000, erg10, erg10B
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

3 bound ligands:
Ligand RB 13 x RB
Ligand CL 4 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 71.26Å b: 105.37Å c: 110.26Å
α: 90° β: 108.69° γ: 90°
R-values:
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli BL21(DE3)

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