5z9b

X-ray diffraction
2.1Å resolution

Bacterial GyrB ATPase domain in complex with (3,4-dichlorophenyl)hydrazine

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES6 (Residues: 15-221; Coverage: 26%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Histidine kinase-like ATPase, C-terminal domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 61.782Å b: 68.231Å c: 102.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.226
Expression system: Escherichia coli