› 5y90

X-ray diffraction
1.3Å resolution

MAP2K7 mutant -C218S

Released:
DOI: 10.2210/pdb5y90/pdb
PDB entry 5y90 coloured by chain, front view.
PDB entry 5y90 coloured by chain, side view.
PDB entry 5y90 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution structure discloses the potential for allosteric regulation of mitogen-activated protein kinase kinase 7.
Kinoshita T, Hashimoto T, Sogabe Y, Fukada H, Matsumoto T, Sawa M
Biochem Biophys Res Commun 493 313-317 (2017)
PMID: 28890347

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 7 Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli B
UniProt:
  • Canonical: O14733 (Residues: 103-419; Coverage: 76%)
Gene names: JNKK2, MAP2K7, MEK7, MKK7, PRKMK7, SKK4
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 52.396Å b: 70.607Å c: 92.155Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.127 0.167
Expression system: Escherichia coli B

Quick links

Citations

1 citations in other articles

Antileukemic properties of the kinase inhibitor OTSSP167 in T-cell acute lymphoblastic leukemia.
Bridges et al. (2023)