› 5wxl

X-ray diffraction
1.9Å resolution

Crystal structure of the Rrs1 and Rpf2 complex

Released:
DOI: 10.2210/pdb5wxl/pdb
PDB entry 5wxl coloured by chain, front view.
PDB entry 5wxl coloured by chain, side view.
PDB entry 5wxl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Molecular architecture of the 90S small subunit pre-ribosome.
Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K
OpenAccess logo Elife 6 (2017)
PMID: 28244370

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153140 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribosome biogenesis protein RPF2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 271 amino acids
Theoretical weight: 31.32 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P36160 (Residues: 19-288; Coverage: 79%)
Gene names: RPF2, YKR081C, YKR401
Sequence domains: Brix domain
Regulator of ribosome biosynthesis Chains: B, D
Molecule details ›
Chains: B, D
Length: 106 amino acids
Theoretical weight: 11.61 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08746 (Residues: 20-121; Coverage: 50%)
Gene names: RRS1, YOR294W
Sequence domains: Ribosome biogenesis regulatory protein (RRS1)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 71.791Å b: 71.791Å c: 268.905Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.21
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Eukaryotic Ribosome Assembly.
Baßler et al. (2019)
23 more

1 mention without citation

Molecular architecture of the 90S small subunit pre-ribosome.
Sun et al. (2017)