› 5meb

X-ray diffraction
1.8Å resolution

Crystal structure of yeast Cdt1 C-terminal domain

Released:
DOI: 10.2210/pdb5meb/pdb
PDB entry 5meb coloured by chain, front view.
PDB entry 5meb coloured by chain, side view.
PDB entry 5meb coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cdt1 stabilizes an open MCM ring for helicase loading.
Frigola J, He J, Kinkelin K, Pye VE, Renault L, Douglas ME, Remus D, Cherepanov P, Costa A, Diffley JFX
OpenAccess logo Nat Commun 8 15720 (2017)
PMID: 28643783

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division cycle protein CDT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 12.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P47112 (Residues: 495-604; Coverage: 18%)
Gene names: CDT1, J1641, SID2, TAH11, YJR046W
Sequence domains: DNA replication factor Cdt1 C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 43.93Å b: 85.87Å c: 89.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.21
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Caught in the act: structural dynamics of replication origin activation and fork progression.
Lewis et al. (2020)
9 more

1 mention without citation

Cdt1 stabilizes an open MCM ring for helicase loading.
Frigola et al. (2017)