5k39

X-ray diffraction
1.98Å resolution

THE TYPE II COHESIN DOCKERIN COMPLEX FROM CLOSTRIDIUM THERMOCELLUM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-107059 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell surface glycoprotein 2 Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 18.95 KDa
Source organism: Acetivibrio thermocellus 27405
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q06853 (Residues: 205-364; Coverage: 24%)
Gene name: Cthe_3079
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like
Dockerin domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 164 amino acids
Theoretical weight: 18.25 KDa
Source organism: Acetivibrio thermocellus 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DGE8 (Residues: 2014-2177; Coverage: 8%)
Gene name: Cthe_1806
Sequence domains: Dockerin type I domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 116.67Å b: 78.63Å c: 35.8Å
α: 90° β: 95.87° γ: 90°
R-values:
R R work R free
0.193 0.187 0.247
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli