› 5f3x

X-ray diffraction
2.65Å resolution

Crystal structure of Harmonin NPDZ1 in complex with ANKS4B SAM-PBM

Released:
DOI: 10.2210/pdb5f3x/pdb
PDB entry 5f3x coloured by chain, front view.
PDB entry 5f3x coloured by chain, side view.
PDB entry 5f3x coloured by chain, top view.
Source organisms:
Primary publication:
Mechanistic Basis of Organization of the Harmonin/USH1C-Mediated Brush Border Microvilli Tip-Link Complex.
Li J, He Y, Lu Q, Zhang M
Dev Cell 36 179-89 (2016)
PMID: 26812017

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185070 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Harmonin Chains: A, C
Molecule details ›
Chains: A, C
Length: 196 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6N9 (Residues: 1-194; Coverage: 35%)
Gene names: AIE75, USH1C
Sequence domains:
Structure domains:
Ankyrin repeat and SAM domain-containing protein 4B Chains: B, D
Molecule details ›
Chains: B, D
Length: 81 amino acids
Theoretical weight: 9.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8K3X6 (Residues: 345-423; Coverage: 19%)
Gene names: Anks4b, Harp
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P32
Unit cell:
a: 124.566Å b: 124.566Å c: 49.654Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.227 0.264
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Regulation of actin-based apical structures on epithelial cells.
Pelaseyed et al. (2018)
7 more