› 5dy6

X-ray diffraction
2.66Å resolution

Enhanced superfolder GFP with DBCO at 148

Released:
DOI: 10.2210/pdb5dy6/pdb
PDB entry 5dy6 coloured by chain, front view.
PDB entry 5dy6 coloured by chain, side view.
PDB entry 5dy6 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle.
Hartley AM, Worthy HL, Reddington SC, Rizkallah PJ, Jones DD
OpenAccess logo Chem Sci 7 6484-6491 (2016)
PMID: 28451106

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 25.94 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A059PIQ0 (Residues: 5-232; Coverage: 91%)
Gene name: gfp
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 42.98Å b: 89.38Å c: 122.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.194 0.29
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Better together: building protein oligomers naturally and by design.
Gwyther et al. (2019)
7 other articles

1 mention without citation

Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle.
Hartley et al. (2016)