› 5c7e

X-ray diffraction
3.1Å resolution

Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal domain (1-209) in complex with Arabidopsis IAA10 peptide

Released:
DOI: 10.2210/pdb5c7e/pdb
PDB entry 5c7e coloured by chain, front view.
PDB entry 5c7e coloured by chain, side view.
PDB entry 5c7e coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors.
Ke J, Ma H, Gu X, Thelen A, Brunzelle JS, Li J, Xu HE, Melcher K
OpenAccess logo Sci Adv 1 e1500107 (2015)
PMID: 26601214

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174367 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein TPR1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 209 amino acids
Theoretical weight: 24.9 KDa
Source organism: Oryza sativa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NBT9 (Residues: 1-209; Coverage: 19%)
Gene names: ASPR2, LOC_Os01g15020, OSNPB_010254100, Os01g0254100, OsJ_01134, P0705D01.10-1, TPR1, TPR2
Sequence domains:
Auxin-responsive protein IAA10 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 11 amino acids
Theoretical weight: 1.16 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: Q38828 (Residues: 41-51; Coverage: 4%)
Gene names: At1g04100, F20D22.13, IAA10

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3121
Unit cell:
a: 162.668Å b: 162.668Å c: 157.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.229
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

Transcriptional Responses to the Auxin Hormone.
Weijers et al. (2016)
21 more

1 mention without citation

A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex.
Ma et al. (2017)