4jpo

X-ray diffraction
5Å resolution

5A resolution structure of Proteasome Assembly Chaperone Hsm3 in complex with a C-terminal fragment of Rpt1

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
hetero tetramer
hetero octamer
PDBe Complex ID:
PDB-CPX-152575 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA mismatch repair protein HSM3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 56.69 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38348 (Residues: 1-480; Coverage: 100%)
Gene names: HSM3, YBR1740, YBR272C
Sequence domains:
26S proteasome regulatory subunit 7 homolog Chains: C, D
Molecule details ›
Chains: C, D
Length: 100 amino acids
Theoretical weight: 11.64 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33299 (Residues: 379-467; Coverage: 19%)
Gene names: CIM5, RPT1, YKL145W, YTA3
Sequence domains: AAA+ lid domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6522
Unit cell:
a: 185.299Å b: 185.299Å c: 357.382Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.253 0.273
Expression system: Escherichia coli BL21(DE3)