› 4gd9

X-ray diffraction
1.5Å resolution

Circular Permuted Streptavidin N49/G48

Released:
DOI: 10.2210/pdb4gd9/pdb
PDB entry 4gd9 coloured by chain, front view.
PDB entry 4gd9 coloured by chain, side view.
PDB entry 4gd9 coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Structural consequences of cutting a binding loop: two circularly permuted variants of streptavidin.
Le Trong I, Chu V, Xing Y, Lybrand TP, Stayton PS, Stenkamp RE
Acta Crystallogr D Biol Crystallogr 69 968-77 (2013)
PMID: 23695241

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 132 amino acids
Theoretical weight: 13.67 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 73-163, 168-72; Coverage: 80%)
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand BTN 4 x BTN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 54.72Å b: 85.89Å c: 100.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.23
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix.
Wu et al. (2017)
2 more

2 mentions without citation

Structural consequences of cutting a binding loop: two circularly permuted variants of streptavidin.
Le Trong et al. (2013)
1 more