4fsx

X-ray diffraction
3.2Å resolution

crystal structure of Se-substituted Zea mays ZMET2 in complex with SAH

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-189610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 784 amino acids
Theoretical weight: 88.34 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AXT8 (Residues: 130-912; Coverage: 86%)
Gene name: MET2A
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 64.857Å b: 88.952Å c: 113.493Å
α: 93.47° β: 95.53° γ: 110.41°
R-values:
R R work R free
0.241 0.24 0.263
Expression system: Escherichia coli BL21(DE3)