4enn

X-ray diffraction
2.84Å resolution

Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-carboxymethylguanine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117429 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Alkyltransferase-like protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 13.66 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UTN9 (Residues: 1-108; Coverage: 100%)
Gene names: SPAC1250.04c, atl1
Sequence domains: 6-O-methylguanine DNA methyltransferase, DNA binding domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
RNA (5'-R(*GP*CP*CP*AP*TP*GP*(C6G)P*CP*TP*AP*GP*TP*A)-3') Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 13 nucleotides
Theoretical weight: 4.05 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P41212
Unit cell:
a: 85.154Å b: 85.154Å c: 150.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.218 0.278
Expression systems:
  • Escherichia coli
  • Not provided