› 4ehs

X-ray diffraction
1.78Å resolution

Crystal structure of Helicobacter pylori DnaG Primase C terminal domain

Released:
DOI: 10.2210/pdb4ehs/pdb
PDB entry 4ehs coloured by chain, front view.
PDB entry 4ehs coloured by chain, side view.
PDB entry 4ehs coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Crystal structure and mode of helicase binding of the C-terminal domain of primase from Helicobacter pylori.
Abdul Rehman SA, Verma V, Mazumder M, Dhar SK, Gourinath S
J Bacteriol 195 2826-38 (2013)
PMID: 23585534

Function and Biology Details

Reaction catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 18.26 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: P56064 (Residues: 413-559; Coverage: 26%)
Gene names: HP_0012, dnaG
Sequence domains: DnaG-primase C-terminal, helicase-binding domain
Structure domains: DNAb Helicase; Chain A

Ligands and Environments

1 bound ligand:
Ligand BME 4 x BME
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 48.883Å b: 61.455Å c: 82.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.249
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A structural view of bacterial DNA replication.
Oakley AJ. (2019)
11 other articles

5 mentions without citation

DnaG Primase-A Target for the Development of Novel Antibacterial Agents.
Ilic et al. (2018)
4 more