› 4d4u

X-ray diffraction
1.99Å resolution

Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with LewisY tetrasaccharide.

Released:
DOI: 10.2210/pdb4d4u/pdb
PDB entry 4d4u coloured by chain, front view.
PDB entry 4d4u coloured by chain, side view.
PDB entry 4d4u coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Structural insights into Aspergillus fumigatus lectin specificity: AFL binding sites are functionally non-equivalent.
Houser J, Komarek J, Cioci G, Varrot A, Imberty A, Wimmerova M
Acta Crystallogr D Biol Crystallogr 71 442-53 (2015)
PMID: 25760594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Fucose-specific lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.63 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WW81 (Residues: 1-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, NAG, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, GAL, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, GAL
Carbohydrate polymer
2 bound ligands:
Ligand GOL 2 x GOL
Ligand FUC 2 x FUC
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 47.317Å b: 47.529Å c: 77.365Å
α: 96.81° β: 100.24° γ: 113.61°
R-values:
R R work R free
0.172 0.169 0.22
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Histo-blood group antigens as mediators of infections.
Heggelund et al. (2017)
1 more

4 mentions without citation

GlyMDB: Glycan Microarray Database and analysis toolset.
Cao et al. (2020)
3 more