› 3wkh

X-ray diffraction
1.64Å resolution

Crystal structure of cellobiose 2-epimerase in complex with epilactose

Released:
DOI: 10.2210/pdb3wkh/pdb
PDB entry 3wkh coloured by chain, front view.
PDB entry 3wkh coloured by chain, side view.
PDB entry 3wkh coloured by chain, top view.
Source organism: Rhodothermus marinus
Primary publication:
Structural insights into the epimerization of β-1,4-linked oligosaccharides catalyzed by cellobiose 2-epimerase, the sole enzyme epimerizing non-anomeric hydroxyl groups of unmodified sugars.
Fujiwara T, Saburi W, Matsui H, Mori H, Yao M
J Biol Chem 289 3405-15 (2014)
PMID: 24362032

Function and Biology Details

Reaction catalysed: 
Cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cellobiose 2-epimerase Chain: A
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 47.46 KDa
Source organism: Rhodothermus marinus
Expression system: Escherichia coli
UniProt:
  • Canonical: F8WRK9 (Residues: 1-412; Coverage: 100%)
Gene name: ce
Sequence domains: N-acylglucosamine 2-epimerase (GlcNAc 2-epimerase)
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, GAL
Carbohydrate polymer
2 bound ligands:
Ligand CL 1 x CL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 41.869Å b: 87.536Å c: 93.962Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.188
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Functions, structures, and applications of cellobiose 2-epimerase and glycoside hydrolase family 130 mannoside phosphorylases.
Saburi W. (2016)
12 other articles

3 mentions without citation

Structural insights into the epimerization of β-1,4-linked oligosaccharides catalyzed by cellobiose 2-epimerase, the sole enzyme epimerizing non-anomeric hydroxyl groups of unmodified sugars.
Fujiwara et al. (2014)
2 more