› 3udu

X-ray diffraction
1.85Å resolution

Crystal structure of putative 3-isopropylmalate dehydrogenase from Campylobacter jejuni

Released:
DOI: 10.2210/pdb3udu/pdb
PDB entry 3udu coloured by chain, front view.
PDB entry 3udu coloured by chain, side view.
PDB entry 3udu coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Tkaczuk KL, Chruszcz M, Grimshaw S, Onopriyenko O, Savchenko A, Anderson WF, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-isopropylmalate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 361 amino acids
Theoretical weight: 39.65 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PLW0 (Residues: 1-358; Coverage: 100%)
Gene names: Cj1718c, leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 74.717Å b: 83.17Å c: 111.204Å
α: 94.19° β: 90.07° γ: 89.64°
R-values:
R R work R free
0.2 0.197 0.236
Expression system: Escherichia coli BL21(DE3)

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