› 3t5t

X-ray diffraction
1.7Å resolution

Vall from streptomyces hygroscopicus in apo form

Released:
DOI: 10.2210/pdb3t5t/pdb
PDB entry 3t5t coloured by chain, front view.
PDB entry 3t5t coloured by chain, side view.
PDB entry 3t5t coloured by chain, top view.
Source organism: Streptomyces hygroscopicus subsp. limoneus
Entry authors: Zhang H, Zheng L, Qian H

Function and Biology Details

Reaction catalysed: 
GDP-valienol + validamine 7-phosphate = validoxylamine A 7'-phosphate + GDP
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Validamine 7-phosphate valienyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 54.88 KDa
Source organism: Streptomyces hygroscopicus subsp. limoneus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15JG1 (Residues: 2-497; Coverage: 100%)
Gene names: SHL15_8006, vldE
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 80.671Å b: 46.693Å c: 123.879Å
α: 90° β: 108.08° γ: 90°
R-values:
R R work R free
0.181 0.179 0.209
Expression system: Escherichia coli

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