› 3qzm

X-ray diffraction
1.25Å resolution

Staphylococcus aureus IsdA NEAT domain H83A variant in complex with heme

Released:
DOI: 10.2210/pdb3qzm/pdb
PDB entry 3qzm coloured by chain, front view.
PDB entry 3qzm coloured by chain, side view.
PDB entry 3qzm coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus N315
Primary publication:
Iron-coordinating tyrosine is a key determinant of NEAT domain heme transfer.
Grigg JC, Mao CX, Murphy ME
J Mol Biol 413 684-98 (2011)
PMID: 21893067

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-regulated surface determinant protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.58 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7A655 (Residues: 62-184; Coverage: 41%)
Gene names: SA0977, frpA, isdA, stbA
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand SO4 2 x SO4
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 52.49Å b: 51.76Å c: 55.69Å
α: 90° β: 91.94° γ: 90°
R-values:
R R work R free
0.14 0.139 0.164
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
3 more