› 3pz5

X-ray diffraction
2.5Å resolution

The crystal structure of AaLeuRS-CP1-D20

Released:
DOI: 10.2210/pdb3pz5/pdb
PDB entry 3pz5 coloured by chain, front view.
PDB entry 3pz5 coloured by chain, side view.
PDB entry 3pz5 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Peripheral insertion modulates the editing activity of the isolated CP1 domain of leucyl-tRNA synthetase.
Liu RJ, Tan M, Du DH, Xu BS, Eriani G, Wang ED
Biochem J 440 217-27 (2011)
PMID: 21819379

Function and Biology Details

Reaction catalysed: 
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.68 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66680 (Residues: 228-439; Coverage: 30%)
Gene names: aq_351, leuS
Sequence domains: Leucyl-tRNA synthetase, editing domain
Structure domains: Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I41
Unit cell:
a: 106.143Å b: 106.143Å c: 81.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.266
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Transfer RNA: a dancer between charging and mis-charging for protein biosynthesis.
Zhou et al. (2013)
1 more

1 mention without citation

Discovery of a potent benzoxaborole-based anti-pneumococcal agent targeting leucyl-tRNA synthetase.
Hu et al. (2013)