› 3k4n

X-ray diffraction
2.75Å resolution

Pyranose 2-oxidase F454A/S455A/Y456A mutant

Released:
DOI: 10.2210/pdb3k4n/pdb
PDB entry 3k4n coloured by chain, front view.
PDB entry 3k4n coloured by chain, side view.
PDB entry 3k4n coloured by chain, top view.
Source organism: Trametes ochracea
Primary publication:
Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase.
Spadiut O, Tan TC, Pisanelli I, Haltrich D, Divne C
FEBS J 277 2892-909 (2010)
PMID: 20528921

Function and Biology Details

Reaction catalysed: 
D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyranose 2-oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 623 amino acids
Theoretical weight: 69.23 KDa
Source organism: Trametes ochracea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7ZA32 (Residues: 1-623; Coverage: 100%)
Gene name: p2o
Sequence domains: GMC oxidoreductase
Structure domains: Phage tail proteins - 2 layer sandwich fold

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P43212
Unit cell:
a: 101.578Å b: 101.578Å c: 249.997Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.191 0.274
Expression system: Escherichia coli

Quick links

Citations

18 citation in other articles

Hydrogen peroxide elimination from C4a-hydroperoxyflavin in a flavoprotein oxidase occurs through a single proton transfer from flavin N5 to a peroxide leaving group.
Sucharitakul et al. (2011)
17 more