› 3gae

X-ray diffraction
1.6Å resolution

Crystal Structure of PUL

Released:
DOI: 10.2210/pdb3gae/pdb
PDB entry 3gae coloured by chain, front view.
PDB entry 3gae coloured by chain, side view.
PDB entry 3gae coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
An Armadillo motif in Ufd3 interacts with Cdc48 and is involved in ubiquitin homeostasis and protein degradation.
Zhao G, Li G, Schindelin H, Lennarz WJ
Proc Natl Acad Sci U S A 106 16197-202 (2009)
PMID: 19805280

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein DOA1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P36037 (Residues: 464-715; Coverage: 35%)
Gene names: DOA1, UFD3, YKL213C, ZZZ4
Sequence domains: PUL domain
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P31
Unit cell:
a: 64.588Å b: 64.588Å c: 115.798Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.215
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Armadillo-repeat protein functions: questions for little creatures.
Tewari et al. (2010)
8 more

2 mentions without citation

An Armadillo motif in Ufd3 interacts with Cdc48 and is involved in ubiquitin homeostasis and protein degradation.
Zhao et al. (2009)
1 more