2x9q

X-ray diffraction
2.02Å resolution

Structure of the Mycobacterium tuberculosis protein, Rv2275, demonstrates that cyclodipeptide synthetases are related to type I tRNA-Synthetases.

Released:

Function and Biology Details

Reaction catalysed:
2 L-tyrosyl-tRNA(Tyr) = 2 tRNA(Tyr) + cyclo(L-tyrosyl-L-tyrosyl)
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-161978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclo(L-tyrosyl-L-tyrosyl) synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 31.9 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPF9 (Residues: 1-289; Coverage: 100%)
Gene name: Rv2275
Sequence domains: Cyclodipeptide synthase
Structure domains: Cyclodipeptide synthase

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 71.09Å b: 75.06Å c: 140.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.21
Expression system: Escherichia coli