2pmc

X-ray diffraction
2.69Å resolution

Crystal Structure of CheY-Mg(2+) in Complex with CheZ(C15) Peptide solved from a P1 Crystal

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-139761 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 14.01 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A2D5 (Residues: 2-129; Coverage: 99%)
Gene names: STM1916, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Protein phosphatase CheZ Chains: E, F
Molecule details ›
Chains: E, F
Length: 15 amino acids
Theoretical weight: 1.62 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
UniProt:
  • Canonical: P07800 (Residues: 200-214; Coverage: 7%)
Gene names: STM1915, cheZ

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 34.76Å b: 53.672Å c: 65.641Å
α: 90.22° β: 102.92° γ: 90.18°
R-values:
R R work R free
0.213 0.205 0.285
Expression systems:
  • Escherichia coli
  • Not provided