› 1rkj

Solution NMR

Solution structure of the complex formed by the two N-terminal RNA-binding domains of nucleolin and a pre-rRNA target

Released:
DOI: 10.2210/pdb1rkj/pdb
PDB entry 1rkj coloured by chain, ensemble of 14 models, front view.
PDB entry 1rkj coloured by chain, ensemble of 14 models, side view.
PDB entry 1rkj coloured by chain, ensemble of 14 models, top view.
Source organisms:
Primary publication:
Solution structure of the complex formed by the two N-terminal RNA-binding domains of nucleolin and a pre-rRNA target.
Johansson C, Finger LD, Trantirek L, Mueller TD, Kim S, Laird-Offringa IA, Feigon J
J Mol Biol 337 799-816 (2004)
PMID: 15033352

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140023 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleolin Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.37 KDa
Source organism: Mesocricetus auratus
Expression system: Escherichia coli
UniProt:
  • Canonical: P08199 (Residues: 299-469; Coverage: 24%)
Gene name: NCL
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(*GP*GP*AP*UP*GP*CP*CP*UP*CP*CP*CP*GP*AP*GP*UP*GP*CP*AP*UP*CP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression.
Maris et al. (2005)
8 more

2 mentions without citation

RNA sequence- and shape-dependent recognition by proteins in the ribonucleoprotein particle.
Stefl et al. (2005)
1 more