› 1np6

X-ray diffraction
1.9Å resolution

Crystal structure of Escherichia coli MobB

Released:
DOI: 10.2210/pdb1np6/pdb
PDB entry 1np6 coloured by chain, front view.
PDB entry 1np6 coloured by chain, side view.
PDB entry 1np6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Insight into the role of Escherichia coli MobB in molybdenum cofactor biosynthesis based on the high resolution crystal structure.
McLuskey K, Harrison JA, Schuttelkopf AW, Boxer DH, Hunter WN
J Biol Chem 278 23706-13 (2003)
PMID: 12682065

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Molybdopterin-guanine dinucleotide biosynthesis adapter protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 19.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32125 (Residues: 2-175; Coverage: 99%)
Gene names: JW5575, b3856, mobB, yihC
Sequence domains: Molybdopterin guanine dinucleotide synthesis protein B
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 52.4Å b: 64.791Å c: 54.241Å
α: 90° β: 97.68° γ: 90°
R-values:
R R work R free
0.218 0.215 0.285
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
4 more